渔民apoB基因3等位基因多态性与血脂水平关系

Polmorphisms of apoB Gene 3′ VNTR Alleles and Analysis of Lipid Level in Fishers

目的 研究海岛地区渔民人群载脂蛋白B(apoB)基因 3′端高可变区等位基因的多态性与血脂水平的关系。方法 采用聚合酶链反应和聚丙烯酰胺梯度凝胶电泳方法分析PCR产物。比较不同基因型人群血脂水平的差异。结果 2 4 7份DNA样品中分离到 10种等位基因 ,其频率分布以HVE32和HVE34最为常见 ,其基因频率为 4 4 33%和 33 6 %。BS基因型人群apoC3及apoc2水平略低于SS基因型人群 ,apoB基因杂合子人群其apoB及apoC2水平高于纯合子人群。结论 渔民人群apoB基因 3′高可变区 (VNTR)等位基因多态性具有基因种类少 。

Objective To study the distribution and frequency of apoB gene 3′ end alleles and try to find if there was any association with the level of plasma lipids.Methods PCR and gradient polyacrylamide gel electrophoresis were used to analysize the chromsome DNA and PCR products.Blood plasma lipids levels among different gene-type groups were also compared.Results Totally 10 alleles were found in the fishers group.The HVE32 and HVE34 were most common,and the frequencies of HVE32 and HVE34 were 44.33% and 33.6%,respectively.The apoC3 and apoC2 levels of VNTR BS group were lower than that of VNTR SS group,the apoB and apoC2 levels of heterozygote group were higher than that of homozygote group.Conclusion The characteristics of polymorphism apoB gene in fishers population included fewer gene type and lower frequency of big genotype,and so on.