青壮年猝死综合征窦房结肽能神经支配的定量分析

A quantitative analysis of peptidergic innervation in sinoatrial node in cases of sudden manhood death syndrome

目的研究青壮年猝死综合征(SMDS)窦房结肽能神经的分布及其比例关系,探讨SMDS的猝死机制。方法采用LSAB免疫组化方法结合计算机图象分析技术,对12例非心性死亡者及6例SMDS窦房结进行神经肽Y(NPY)、血管活性肠肽(VIP)的免疫组化染色并作定量分析。结果SMDS组与非心性死亡组相比,窦房结NPY、VIP免疫阳性物含量及VIP/NPY两者比值均无显著性差异。结论SMDS的猝死机制可能与心脏自主神经功能失调无明显相关。

Objective To study the distribution and proportion of neuropeptide containing nervers in the sinus node in cases of sudden manhood death syndrome(SMDS)and to explore the mechanism of SMDS.Methods Immunohistochemical staining and quantitative analysis of neuropeptide Y(NPY)and vasoactive intestinal peptide(VIP)in the sinus node in6cases of SMDS and in12cases of non-cardiac death(control group)were achieved by LSAB method and computerized image system.Results As for NPY positive materials,VIP positive materials and the ratio of VIP/NPY in the sinus nodes,there were no significant difference between the control group and SMDS group.Conclusion The mechanism of SMDS and the abnormality of autonomic nervous innervation in the sinoatrial nodes maybe incorrelation.