大鼠闭合性小脑挫伤细胞凋亡研究

Cell apoptosis in closed cerebellar contusion in rat

目的探讨闭合性小脑挫伤细胞凋亡发生发展过程。方法采用DNA缺口末端标记法比较SD大鼠闭合性小脑挫伤不同时间损伤区和正常对照组小脑神经细胞凋亡情况。结果在伤后1h发现挫伤区少数凋亡细胞,4～6h达高峰,随后下降。在挫伤缘区域伤后1h亦发现少数散在的凋亡细胞,随着损伤时间延长,凋亡细胞数量增多,在6～8h达到高峰。结论闭合性小脑挫伤不同损伤时间细胞凋亡指数变化为法医学早期损伤时间推断提供了灵敏客观的实验依据。

Objective To observe the cell apoptosis explore closed cerebellar contusion in rat.Methods SD rats model of contusion was established and apototic cells were detected by TUNEL method at5,10,30,45,60min and2,4,6,8,12h,after injury.Results apototic cells in contusion area appeared at1h after closed cerebellar contusion,reached the peak at4-6h,then decreased and so as marginal area where the peak was in6-8h.Conclusion The apoptotic index of closed contusion of cerebellum in different injury time may provide a new sensitive and objective method for the forensic early injury time estimation.