复合维生素对小鼠免疫功能的影响

INFLUENCE OF VITAMIN COMPLEX ON IMMUNE FUNCTION IN MICE

本实验研究了维生素A、E、B_2、B_6及叶酸等供给不足及加倍补充后对BALB/C小鼠免疫功能的复合效应。动物喂养6周(恢复组喂8周)后取材实验。结果表明:不足组外周血T细胞、颗粒型T细胞百分率比对照组显著降低,补充正常量维生素后两者恢复正常,而2倍及3倍量组则比对照组显著升高。弥散型T细胞各组无差异。胸腺相对及绝对重量与T细胞百分率呈显著正相关。不足组脾抗体形成细胞比对照组显著减少,补充正常量维生素后抗体形成细胞显著增加,而2倍和3倍量组脾抗体形成细胞,ConA、PHA及LPS刺激后脾淋巴细胞~3H-TdR掺入率也比对照组显著升高(不足组脾淋巴细胞~3H-TdR掺入率不升高)。实验还显示,该复合维生素对脾组织cAMP和cGMP含量均有显著影响,这可能是其作用于免疫系统的生化机制。

The influence of individual vitamin on immunity has been described before. In this report, the comprehensive effect of vitamin A, E, riboflavin, pyridoxine and folic acid on the immune response was studied in the BALB/C mice. Animals were divided into five groups:control group, one-third requirement group, double requirement group, triple requirement group and restored group (feeding insufficient diet for six weeks, and then normal diet for another two weeks). The results showed that the percentages of the peripheral blood T cell, Tu cell were diminished significantly in the deficient group in comparison to controls, on the contrast, those in double and triple requirement groups increased obviously, and also did the restored group supplemented normally but no difference in the percentage of Ty in all groups. The study also showed a positive correlation between the relative, absolute thymic weight and the percentage of the peripheral blood T cell. The changes stated above of the peripheral blood T cell and its sub-populations are partially owing to the abnormality of the thymic tissue, but the distribution of lymphocyte shouldn't be neglected. The study found the plaque forming cells (PFC) of spleen, aud the ratio of 3H-thymidine incorporating into splenic lymphocyte after exposure to ConA, PHA and LPS in vitro were increased significantly in double and triple requirement groups; but with the vitamin complex under supply, the former was requced significantly, the latter was comparable to the controls. The PFC increased markedly and no difference with the double group after insufficient animals fed normal diet for two weeks. It is therefore not surprising that the vitamin complex plays a special role in the differentiation and maturation of PFC. According to the study, the vitamin complex may influence the growth of lymphoid tissue, the differentiation and maturation of immunocompetent cells and functional manifestation by mainly affecting the contents of cAMP, cGMP of lymphocyte, and the metabolism of nucleic acid and protein.