一种新型低密度脂蛋白吸附剂的制备

Preparation of a New Adsorbent for Binding Low Density Lipoprotein

通过在环氧化的交联聚乙烯醇球上固载多乙烯多胺和磺酸基团的方法制备一种新型的低密度脂蛋白吸附剂 .环氧化的交联聚乙烯醇球首先与乙二胺或二乙烯三胺、三乙烯四胺及四乙烯五胺等多乙烯多胺进行胺化反应 ,由此获得不同长度悬臂 ,而后将胺化的环氧化的交联聚乙烯醇球与 DMF/ Cl SO3H反应 ,从而固载磺酸基团 .磺酸基团的固载量随交联度的减小和聚乙烯醇浓度的增大而增大 .初步研究证实 。

A new adsorbent for binding low density lipoprotein was prepared by the immobilization of sulfuric group on crosslinked poly(vinyl alcohol) bead. Spacers with different lengths were introduced, by the reaction with polyethylene polyamine, to the crosslinked poly(vinyl alcohol) bead with epoxy group, and then sulfuric group was immobilized by the reaction with DMF/ClSO 3H. It was found that this method could increase the amount of immobilized sulfuric group, which increased with decreasing crosslinking degree and increasing PVA concentration. The adsorption experiments in vitro showed that this kind of adsorbent had a satisfactory adsorption capacity and selectivity.