5World Health Organization. WHO laboratory manual for the ex- amination and processing of human semen [M]. 5th ed. Geneva: WHO Press, 2010 : 16.
6Li Y, Lin H, Li Y, et al. Association between socio psycho-behav ioral factors and male semen quality:systematic review and meta- analyses[J]. Fertil Steril,2011,95(1) :116- 123.
7Martini AC, Molina RI, Estofan D, et al. Effects of alcohol and cigarette consumption on human seminal quality[J]. Fertil Steril, 2004,82(2) :374-377.
8Kumar S,Kumari A, Murarka S. Lifestyle factors in deteriorating male reproductive health[J]. Indian J Exp Bid, 2009,47 (8) : 615- 624.
9Meseguer M, Garrido N, Martinez-Conejero JA, et al. Relation- ship between standard semen parameters, Calcium, cholesterol contents, and mitochondrial activity in ejaculated spermatozoa from fertile and infertile males[J]. J Assist Reprod Genet,2004, 21(12) :445-451.
10Wennemutb G, Babcock DF, Hille B. Calcium clearance mecha- nisms of mouse sperm[J]. J Gen Physiol,2003,122(1) :115-128.