心脉神口服液对低密度脂蛋白介导的内皮细胞分泌一氧化氮和内皮素-1的影响

Effect of Xinmaishen Oral Liquid on Ox_LDL_induced Decrease of NO and Increase of ET-1 Release from Human Endothelial Cells

目的探讨中成药心脉神口服液 (XMSol)对氧化型低密度脂蛋白 (ox_LDL)致血管内皮细胞(VEC)损伤的细胞保护作用。方法进行人脐静脉内皮细胞 (HUVEC)体外培养 ;用中药血清药理学的方法制备心脉神口服液 (XMSol)含药血清 ;用放射免疫法等技术观察ox_LDL对VEC分泌一氧化氮 (NO)及内皮素 -l(ET-1)的影响并研究心脉神口服液 (XMSol)的保护作用。结果培养液中加有ox_LDL的VEC分泌的NO显著降低 ,分泌的ET-1明显升高。于加入ox_LDL之前预先加入XMSol或同时加入XM Sol时 ,VEC分泌NO较单用ox_LDL明显升高 ,ET-1明显较低。结论心脉神口服液能拮抗ox_LDL致内皮细胞分泌NO下降和ET-1升高。

Objective To investigate the injurious effects of oxidized LDL (ox_LDL) on human endothelial cells(VEC) and protective effects of Xinmaishen oral liquid(XMSol).Methods The effects of ox_LDL on nitric oxide(N0), endothelin_1(ET-1) in cultured human umbilical venous endothelial cells(HUVECs) and the protective role of XMSol were observed.Results Ox_LDL led to a decrease of No release and an increase of ET-1 Which can be reversed by XMSol. Conclusions XMSol has antagonistic effects on ox_LDL_induced decreasing of No and increasing of ET-1 on HUVECs.