终末器官对端侧神经吻合后轴突再生影响的实验研究

The influence of end organs on peripheral nerve regeneration after end-to-side neurorrhaphy: an experiment study

目的 探讨受神经远端终末器官对端侧吻合后神经再生的影响。方法 SD雌性大鼠 1 0只 ,动物随机分为A、B2组。A组 :在近端切断腓总神经 ,其远断端与胫神经外膜开窗处进行端侧吻合 ;B组 :同A组两神经端侧吻合后 ,在距吻合口 1 .5cm处切断其与终末器官的联系 ,并将连接肌肉的腓总神经远端反折固定在邻近的肌肉组织。术后 1 2周取吻合口处的神经标本 ,甲苯胺蓝和抗神经微丝抗体免疫组化染色、光镜检查及图像分析。结果 甲苯胺蓝染色及神经微丝免疫组化染色结果说明A、B2组再生神经纤维数目、髓鞘厚度、纤维结缔组织含量及神经纤维丝排列 ,均有明显差异。

Objective To investigate the influence of end organs on peripheral nerve regeneration after end -to- side neurorrhaphy. Methods 10 female adult SD rats were randomly divided into two groups: group A, The distal end of transected common peroneal nerve on the left side, was sutured through an epineurial window in end -to-side fashion; group B, The left common peroneal nerve was sutured to the tibial nerve in the same fashion as the group A ,but the peroneal nerve was cut off 1.5cm distance to the neurorrhaphy point. The distal peroneal segment, which was connected with the peroneal muscle, was inversed and fixed to the nearby muscle. The regeneration was tested via methylamine blue stain and neuro filament immunohistochemistry 12 weeks postoperation. Results There are obviously differences between the acceptive nerves of A and B groups in view of the counts of the regenerated axon, the width of the myelin sheath, the amounts of the fibrous connected tissue and the arrangement of the regenerated nerve fiber. Conclusion The end organs are important factors to the peripheral nerve regeneration for end-to-side neurorrhaphy.