利用失血性贫血动物模型评价含EPO因子功能食品的方法

Using Blood Loss Anemia Rat Model for Evaluating EPO Factor Containing Food

建立一种评价含EPO因子功能食品的实验方法。方法:饲喂低铁饲料及内眦静脉丛放血建立大鼠失血性贫血模型,以红细胞数及血红蛋白含量为检测指标,观察口服转红细胞生成素(Erythropoietin,简称EPO)基因蚕蛹表达产物对失血性血模型大鼠的影响。结果:利用失血性贫血模型,通过检测血液红细胞数量及血红蛋白含量,能够将转EPO基因蚕蛹表达产物促进红细胞生成和加速血红蛋白合成的作用准确地反映出来。结论:用这一方法对含EPO因子的食品进行功能评价是可行的。

Objective:Anexperimental method f or evaluating the erythropoietin(EPO)factor containing food was es-tablished.Methods:Through a low ir on containing food and vein nomospasia a blood loss anemia rat model was se t up.The effect of the EPOfactor conta ining food was identified by inspection of the number of red cells and cont ent of hemoglobin,the feasibility of the m ethodwas testedby inspectionofthe number ofredcells andcontentofhem oglobin,thefeasibilityofthemethodwastestedbyexaminingtheeffectofanoraladministrationofthehumanEPOgene expression productinsilkwormchrysalises.Re sults:Usingthebloodloss anemiamo del,throughthenumberofredcellsandcontent ofhemoglobininspection,the effectof trans -EPO -gene product gave a go od effect ofpromoting the productio n of red cells and expediting the synthesis of hemoglobin.Conclusion:The intro duced methods can be used for evaluating the EPOfactor containing foods.