棉花黄萎病菌营养体亲和群的RAPD图谱特征

RAPD Patterns Characteristic for VCGs of Cotton Verticillium Wilt Pathogen

条引物对 10 3个Verticilliumdahliae菌株进行RAPD扩增 ,根据RAPD图谱计算出的菌株间遗传相似性进行聚类分析 ,可将棉花黄萎病菌分为 2组 (RPG1,RPG2 )。RPG1包括了所有VCGI菌株 ,RPG2包括VCGⅢ和VCGⅣ等 2个营养体亲和群 ;RPG1内菌株间遗传差异较小 ,遗传变异量为 0 .0 996 ;RPG2内菌株间遗传差异较大 ,遗传变异量是 0 .15 92。对已划分RPG而其营养体亲和群归属未知的 15个菌株进行营养体亲和性测定验证 ,结果表明 ,除 1个菌株 (V4 93)外 ,归在RPG1的菌株都属于VCGI,归入RPG2的菌株均属于VCGⅢ。有 5条RAPD扩增带在VCGI内的出现频率大于 0 .96 ,而其在VCGⅢ的出现频率均小于 0 .1,这 5条带可以看作VCGI的特征条带 ;

Nine primers were used to amplify DNA of all 103 Verticillium dahliae isolates used in this study. Based on the genetic distance from the result of RAPD fingerprinting, all isolates which had been tested the vegetative compatibility (VC) were divided into two groups. The first one, RPG1, consisted of isolates belonging to vegetative compatibility group I (VCGI); the second one, RPG2, consisted of isolates belonging to VCGⅢ and VCGIV. There were more genetic diversities in VCGⅡ than in VCGI. The VC examination was done for 15 isolates whose RPGs were made but the VCGs were unknown. Results indicated that isolates falling into RPG1 belonging to VCGI and isolates falling into RPG2 belonging to VCGⅢ, except for one isolate (V493). Five RAPD fragments, with frequencies more than 0.96 in VCGI but less than 0.1 in VCGⅢ, could be identified as distinctive fragments for VCGI (defoliating type). RAPD results revealed that there was strong correlation between the RPGs and the VCGs.