混合重金属离子诱导的鲫鱼肝脏DNA的断裂与修复

The liver cellur DNA breaks and repair of the fish (carassius auratus) induced by the sublethal metal mixture

采用凝胶电泳技术和3 H -TdR掺入实验研究了亚致死浓度的混合重金属离子诱导的鲫鱼肝脏细胞DNA断裂与修复作用。结果表明 :Zn、Pb和混合重金属离子引发了鲫鱼肝脏细胞DNA的断裂 ,相对断裂数大小顺序为混合重金属离子 >Pb >Zn ;Cd、Zn、Pb和混合重金属离子的处理中都检测到修复作用的存在 ,修复作用的大小顺序为混合重金属离子 >Pb >Zn >Cd ;重金属离子混合后 ,对鲫鱼肝脏细胞DNA的损伤作用增大 ,但DNA的断裂和修复结果不完全相同。

The breaks and repair of the carassius auratus's cellur DNA induced by the metal mixture were studied by using the agrose gel elecrophrosis technique and 3H-TdR incorporation experiment. The results demonstrated that DNA breaks were detected after treated by zine, lead and metal mixture, and the RNSB order was the metal mixture>Pb>Zn. The DNA repair was found among the zinc treatment, cadmium treatment, lead treatment as well as metal mixture treatment, and the order of the repair ability was the metal mixture>Pb>Zn>Cd. The mixture of the metals increased the damage to the DNA. The results also showed that the result of the DNA breaks was not conformed completely to that of the DNA repair.