过期妊娠胎盘和子宫肌层雌、孕激素受体表达的免疫组化研究

Immunohistochemical study on estrogen and progesterone receptors in myometrial and placental tissues in prolonged pregnancy

目的 对胎盘和子宫肌层雌激素受体 (ER)及孕酮受体 (PR)的表达进行检测 ,探讨ER及PR与过期妊娠的关系。方法 采用免疫组化法结合计算机辅助图像分析技术 ,定量分析过期妊娠胎盘和子宫下段肌层组织ER及PR表达。结果 ①胎盘合体滋养细胞及子宫平滑肌细胞均有ER和PR的表达。②过期妊娠子宫下段肌层PR含量高于足月妊娠者 (PU值 9 2 2± 0 72vs 8 5 6± 0 68) ,ER含量低于足月妊娠者 (PU值 8 3 6± 0 73vs 10 5 3± 0 5 7)。过期妊娠胎盘和子宫下段肌层ER/PR比值均低于足月妊娠者 (PU值分别为 1 2 9± 0 11vs 1 41± 0 16,0 91± 0 10vs 1 2 2±0 14 )。结论 胎盘和子宫下段肌层ER/PR比值低下与过期妊娠的发生有关。

Objective To determine whether prolonged pregnancy was associated with changes of estrogen and progesterone receptors in placental and myometrial tissues. Methods Immunocytochemistry and morphometry were used to determine the expression and quantitative evaluation of estrogen and progesterone receptors in the lower myometrial segment and placental tissues obtained from women with prolonged pregnancy. Results Myometrium and placenta from pregnant women contained estrogen and progesterone receptors. These receptors were present in placental syncytiotrophoblasts and myometrial smooth muscle cells. The immunostaining intensity for progesterone receptor was higher in myometrium in the women with prolonged pregnancy than those with term pregnancy. On the contrary, the estrogen receptor was lower in prolonged pregnancy. Simultaneously, the estrogen-progesterone receptor ratios in myometrium and placenta were lower in prolonged pregnancy than those in term pregnancy. Conclusion Decrease in estrogen receptor-progesterone receptor ratios in myometrial and placental tissue may play a role in the occurrence of prolonged pregnancy.