后发性白内障形成中晶体上皮细胞PCNA的表达

Expression of PCNA in lens epithelial cells during the formation of after cataract

探讨后发性白内障形成过程中晶体上皮细胞的增殖性变化。方法 :采用免疫组化SABC法对兔后发性白内障形成过程中晶体上皮细胞PCNA的表达进行检测。结果 :①术后各组增殖指数 (PI值 )均大于对照组 ,除术后 5个月时赤道部P =0 0 1外 ,其余均P <0 0 1。②术后第 1天晶体上皮细胞PCNA表达明显增强 ,赤道部PI值为 0 6 1± 0 0 6 ,前囊下部为 0 5 5± 0 0 5 ,与对照组相比P <0 0 1;术后 1天 ,后囊出现PCNA阳性细胞 ;术后 2个月后囊纤维化。③赤道部PCNA阳性表达强于前囊下部 ,术后 1天、 3天、 1个月与前囊下相比P <0 0 1,术后 1周、 2～ 5个月P >0 0 5。结论 :晶体上皮细胞的增殖、移行发生在术后早期 ,术后 1天增殖能力最强 ;赤道部细胞增殖能力强于前囊下部 ;术后晶体上皮细胞移行、增殖、分化导致后发性白内障的形成。

Objective:To determine the proliferative changes in lens epithelial cells (LECs) during the formation of after cataract. Methods:The expression of proliferation cell nuclear antigen (PCNA) in LECs during the after cataract formation of rabbits was detected with SABC immunohistochemical methods. Results:Postoperative proliferation indexes were great (P<0.05).On the first day after operation,the expression of PCNA in LECs was obviously increased,and positive PCNA cells in posterior capsule were also found,but the fibrosis of posterior capsule was shown at the end of the second month after operation.The positive PCNA expression in the equatorial part was greater than that in subcapsular part of anterior capsule at many time stages within the first month after operation (P<0.01). Conclusions:It seems in the early postoperative stage that the proliferation of LECs occurs,even on the first day after operation.The proliferation activity in equatorial part seems greater than that in subcapsular part of anterior capsule.