17997眼膏对兔实验性单纯疱疹角膜炎的疗效观察

Efficacy of 17997 ointment against experimental herpes simplex keratitis in rabbits

目的 评价不同浓度 17997眼膏治疗实验性单纯疱疹病毒性角膜炎 (herpes sim -plex keratitis,HSK)的疗效及其刺激性。方法 建立兔眼 HSK动物模型 ,给予 5 0、2 0 0 μg·g- 1 17997眼膏滴眼 ,并以 10 g· L- 1无环鸟苷眼膏、赋形剂和空白组作为对照 ,裂隙灯观察角膜病变并评分 ,分离病毒 ,计算病毒滴度评价 17997眼膏疗效。采用 Draize眼刺激实验评价药物毒性。结果 5 0 μg· g- 1 17997眼膏角膜病变评分及平均病毒滴度均明显增高 ,与赋形剂和空白组统计学上无显著性差异 (P>0 .0 5 )。 2 0 0 μg· g- 1 17997眼膏组与 10 g·L- 1无环鸟苷眼膏对照组角膜病变相似 ,病毒滴度高峰时平均病毒滴度升高不明显 ,2组统计学上无显著性差异 (P>0 .0 5 ) ,与另外 3组统计学上有显著性差异 (P<0 .0 5 )。 17997眼膏的 Draize评分与赋形剂组统计学上无显著性差异 (P>0 .0 5 )。结论 5 0 μg· g- 1 17997眼膏对 HSK治疗无效 ;2 0 0μg· g- 1 17997眼膏能有效地治疗 HSK,疗效与 10 g· L- 1 无环鸟苷眼膏相似 。

Objective To evaluate the efficacy and toxicity of different concentration of 17997 ointment against experimental herpes simplex keratitis(HSK) in rabbits.Methods Rabbit eye model of HSK was constructed. 50? 200μg·g -1 17997 ointment was given in rabbit eyes respectively. 10g·L -1 ACV ointment, vehicle and blank were used as control groups. The severity of HSK was evaluated by slit lamp examinations and was scored. Virus was isolated and TCID50 (tissue culture infective dose) was calculated. Draize irritation test was applied to evaluate the eye toxicity of 17997 ointment in vivo. Results The results showed higher keratitis scores and higher average virus titers in 50μg·g -1 17997 ointment group. There was no statistical significance among the blank group, vehicle group and 50μg·g -1 17997 ointment group( P >0.05). The reduction in the severity of HSK in 200μg·g -1 17997 ointment group was similar to that in 10g·L -1 ACV ointment group, lower titers was demonstrated in the fastigium. There was no statistical significance between the two groups( P >0.05). Compared with the other three groups, there was statistical significance among them ( P <0.05). The experiment of stimulation showed that no toxic reaction was observed as Draize score criterion. There was no statistical significance in any groups( P >0.05). Conclusion 50μg·g -1 17997 ointment had no effect on HSK, 200μg·g -1 17997 ointment, as the same as 10g·L -1 ACV ointment, has a strong inhibitory effect against HSV 1 virus, and no toxicity.