幽门螺杆菌感染儿童胃十二指肠粘膜IL-8含量的研究

Interleukin-8 levels in gastroduodenal mucosa in children with Helicobacter pylori infection

检测幽门螺杆菌 (H.pylori)感染儿童的胃、十二指肠粘膜中IL_8含量的变化 ,以探讨其在H.pylori相关性胃十二指肠疾病中的机理。在胃镜下取胃窦及十二指肠球部粘膜活检标本 ,用ELISA法测定粘膜中IL_8的含量。结果 :H.pylori阳性者胃粘膜IL_8为 (24.66～177.77)pg/mg,H.pylori阴性者为(2.94～12.98)pg/mg,两者相比t=12.34,P<0.01 ;H.pylori阳性者十二指肠粘膜IL_8为(12.98～177.77)pg/mg,H.pylori阴性者为(2.04～10.43)pg/mg,两者相比t=7.18,P<0.01。活动性胃炎胃粘膜IL_8为(12.98～177.77)pg/mg,非活动性胃炎为(2.04～10.43)pg/mg,两者相比t=10.66,P<0.01;活动性胃炎十二指肠粘膜IL_8为(5.28～47.76)pg/mg ,非活动性胃炎为(3.19～8.14)pg/mg ,两者相比t=6.52,P<0.01。说明H.pylori阳性和活动性胃炎胃粘膜及十二指肠粘膜IL_8含量均较高。提示H.pylori感染时 ,IL_8在胃十二指肠粘膜局部大量中性粒细胞浸润中可能有重要作用。

To explore the possible role of interleukin_8(IL_8)levels in gastroduodenal mucosa in the pathogenesis of gastroduodenal diseases induced by Helicobacter pylori(H.pylori)infection,interleukin_8 content in gastroduodenal mucosa collected by gastroscopy was determined by using ELISA in 36 children with gastritis induced by either H.pylori or non_H.pylori infection.The results showed that both of IL_8 levels in gastric mucosa and in duodenal mucosa were 24.66～177.77pg/mg and 12.98～177.77pg/mg in children with H.pylori infection and 2.94～12.96 pg/mg and 2.04～10.43 pg/mg in children without H.pylori infection,respectively(t=12.34,P<0.01;t=7.18.P<0.01).Furthermore,both of IL_8 levels in gastric mucosa and in duodenal mucosa were 12.98～177.77pg/mg and 5.28～47.76 pg/mg in children with active gastritis and 2.04～10.43pg/mg and 3.19～8.14 pg/mg in children without active gastritis, respectively (t=10.66, P<0.01;t=6.52,P<0.01).It is indicated that there is a significant increase of IL_8 level in gastroduodenal mucosa in children with either Hp infection of active gastritis,suggesting IL_8 play an important role in the pathogenesis of gastroduodenal diseases that there usually is the infiltration of a large number of neutrophils in local gastroduodenal mucosa in children with H.pylori infection.