箭根薯的试管繁殖

In vitro propagation of Tacca chantrieri

本实验用箭根薯成熟种子为材料 ,培育出无菌幼苗 ,再用幼叶、叶柄进行试管繁殖。经实验得出各阶段适宜的培养基分别为 :(1 )种子萌发 :MS+6BA1 mg/L (单位下同 ) +NAA0 .1 ;(2 )诱导愈伤组织 :MS+6BA1 +2 ,4D1 .5 +KT0 .2 ;(3 )丛芽诱导 :MS+6BA0 .5 +NAA0 .5 ;(4 )生根培养 :1 /2 MS+NAA0 .5。

Tacca chantrieri bear many seeds,but only few of the seeds (about 12%) can germinate,so tissue culture would be a good means for multiplying plants of T.chantrieri. In this experiment,young leaves and leaf stalks,obtained from mature T.chantrieri seeds,were used as explants for in vitro propagation.The appropriate media for respective culture stages were as follows.The medium of seed germinarion was MS+6 BA 1mg/L+NAA0 1mg/L,that of callus inducing was MS+6BA1mg/L+2,4D1 5mg/L+KT0 2mg/L,that of shoot inducing was MS+ 6BA 0 5mg/L+NAA 0 5 mg/L,that of rooting was 1/2 MS+NAA 0 5 mg/L.