用离子色谱法测定抗生素药物中N-甲基吡咯烷的含量

The determination of the content of N-methyl-pyrrolidine in cefepime antibiotic drugs by ion chromatography

用离子色谱法成功地取代液相色谱法而建立了测定Cefepime抗生素药物中N-甲基吡咯烷含量的新方法。用DX-120离子色谱仪,以50mmol/L甲基磺酸为流动相,可测定药物中N-甲基吡咯烷的含量。对同一样品分析六次,其峰面积的相对标准偏差小于1.0％。标准曲线的相关系数=0.9996。

A new method to detennine the content of N-methyl-pyrrolidine by using ion chromatography was developed. The DX-120 ion chromatograph with 50mmol/L methanesulfonic acid as the eluent can be used to determine the content of N-methyl-pyrrolidine in cefepime antibiotic drugs. The RSD is less than 1.0% , and the correlation coefficient of the calibration curve = 0.9996.