一氧化氮对大鼠实验性肝纤维化的影响

Effects of nitric oxide on experimental hepatic fibrosis in rats

目的 研究一氧化氮 (NO)在肝纤维化发病中的作用。方法 建立大鼠肝纤维化模型 ,给予NO前体———L 精氨酸 (L Arg)及一氧化氮合酶 (NOS)抑制剂———硝基 L 精氨酸 (L NNA) ,应用病理组织学检查、放免法及生化学方法 ,观察其对肝纤维化程度、透明质酸 (HA)含量、谷 草转氨酶 (AST)及谷 丙转氨酶 (ALT)活性的影响 ,同时测定NO、一氧化氮合酶 (NOS)水平变化。结果 NO能明显降低肝纤维化程度、HA含量、AST及ALT活性。

AIM To study the role of nitric oxide (NO) in the pathogenesis of hepatic fibrosis. METHODS To establish the model of hepatic fibrosis in rats and treated with L Arg (preNO) and L NNA (NOS inhibitor). The degree of hepatic fibrosis, the levels of HA,AST,ALT determined with histology,radioimmunoassay, autochemol analysis, respectively. NO and NOS were also determined. RESULTS The degree of hepatic fibrosis, the levels of AST,ALT and HA in L Arg group were all lower than those in hepatic fibrosis control group. While the levels of HA and AST in L NNA group were higher than that in hepatic fibrosis control group. CONCLUSION NO has the function of protecting liver cells and anti hepatofibrosis liver fibrosis induced by CCl 4 in rats.