应用组织芯片技术研究肝癌与乙肝病毒感染的关系

Study of Relationship Between Hepatocellular Carcinoma and HBV Infection with the Use of Tissue Chip

目的 研究肝细胞性肝癌 (HCC)与乙肝病毒感染的关系。方法 制作包含 40例肝细胞性肝癌及相应的癌旁和非癌组织的组织芯片。以免疫组织化学方法 (SP法 )检测 40例肝癌组织及其相应的癌旁组织及非癌组织中的 HBs Ag和 HBc Ag表达情况。结果 40例肝癌组织及其相应的癌旁组织和非癌组织中的 HBs Ag检出率分别为 7.5 %、40 %和 45 %;HBc Ag的检出率分别为 2 0 %、5 2 .5 %和 6 7.5 %;HCC肿瘤组织中的 HBs Ag和HBc Ag的表达率明显低于癌旁及非癌组织 (P<0 .0 1)。结论 HCC的发生与 HBV感染有密切关系 ,随着 HCC的发展 ,HBV抗原的表达降低。组织芯片技术是可高效进行肿瘤分子病理研究的技术平台 ,能够在严格、均一的实验条件下 ,高通量、快速、平行地检测大批量组织样本中的一个或多个实验指标。

Objective To study the relationship between hepatocellular carcinoma (HCC) and HBV infection. Methods The tissue chips which contained tumor tissues corresponding adjacent liver tissues and normal liver tissues of 40 cases of HCC were constructed. The expression of HBsAg and HBcAg proteins in tumor tissues, corresponding adjacent liver tissues and normal liver tissues of 40 cases of HCC was studied with SP immunohistochemical technique.Results The positive rate of HBsAg in tumor tissues of 40 HCCs was 7.5%; in adjacent liver tissues, 40%; in normal liver tissues, 45%. The positive rate of HBcAg in tumor tissues of 40 HCCs was 20%; in adjacent liver tissues, 52.5%; in normal liver tissues, 67.5%. The expression of HBsAg and HBcAg in tumor tissues was significantly lower than that in adjacent liver tissues and normal liver tissues(P<0.01). Conclusion HCC carcinogenesis is related to HBV infection. As HCC develops, the expression of HBV antigen decreases. Tissue chip is an effective high-throughput technique platform for researches on tumor molecular pathology.