冠心病患者血清HDL亚类组成的研究

Study on the Content of Serum HDL Subclasses in Patients with Coronary Heart Disease

目的 研究冠心病患者 HDL亚类组成及相对含量的变化。方法 采用双相电泳 -免疫印迹法分析了 83例冠心病患者及 72例正常人血清 preβ- HDL及α- HDL各亚类含量。结果 冠心病患者血清中 preβ1 -HDL ,HDL3 b,HDL3 c水平显著升高 (P<0 .0 0 1) ,HDL2 b显著减少 (P<0 .0 0 1)。冠心病患者中男性 preβ1 - HDL及HDL3 b水平显著高于女性 (P<0 .0 5 ) ,HDL2 b含量则显著低于女性 (P<0 .0 1)。正常对照中男性 HDL3 b显著高于女性 (P<0 .0 5 ) ,preβ1 - HDL 较女性有升高趋势 ,HDL2 b较女性有减少趋势。冠心病患者血清中 TG浓度与 preβ1 -HDL (P<0 .0 1) ,HDL3 b(P<0 .0 1)显著正相关 ,与 HDL2 b(P<0 .0 1)显著负相关。 HDL- C水平与 HDL2 b(P<0 .0 1) ,HDL2 a(P<0 .0 5 )显著正相关 ,与 preβ1 - HDL (P<0 .0 1)显著负相关。apo A 水平与 HDL- C水平与 HDL2 b(P<0 .0 1) ,HDL2 a(P<0 .0 5 )显著正相关 ,与 HDL3 a(P<0 .0 1)显著负相关。结论 冠心病患者血清中 preβ1 - HDL,HDL3 b,HDL3 c水平显著升高 ,HDL2 b显著减少 ,提示其 HDL 颗粒有变小的趋势。而且正常人 ,特别是冠心病患者男性 HDL颗粒较女性为小 ,提示性别差异可能与血清 HDL亚类组成有关。

Objective To study the change of the contents of serum HDL subclasses in subjects with coronary heart disease (CHD). Methods Contents of serum HDL subclasses in CHD subjects (n=83) and healthy controls (n=72) were determined by two dimensional gel electrophoresis associated with immunodetection method. Results In CHD subjects the contents of pre β 1-HDL, HDL 3b, HDL 3c (P<0.001) increased significantly and that of HDL 2b (P<0.001) decreased significantly as compared with those in healthy controls. The levels of pre β 1-HDL, HDL 3b were significantly higher (P<0.05) and HDL 2b level was significantly lower (P<0.01) in male CHD subjects than in female CHD subjects. In control group, the level of HDL 3b(P<0.05 ) was significantly higher in males than in females. In CHD subjects, serum TG concentration showed positive correlation with pre β 1-HDL (P<0.01), HDL 3b(P<0.01) and negative correlation with HDL 2b(P<0.01). Conclusion The significantly higher level of serum pre β 1-HDL, HDL 3b, HDL 3c and lower level of HDL 2b indicated a general shift of CHD subjects' particle size of HDL toward smaller size and that the maturation of HDL could be abnormal in CHD subjects. The particle size of HDL found to be smaller in both CHD and control male subjects than in female subjects suggested that sex seemed to be related to the contents of serum HDL subjects.