胰腺癌组织中c-erbB-2、nm23-H1癌基因及PCNA的表达及意义

Expression of c-erbB-2,nm23-H1 proteins and PCNA in pancreatic adenocarcinoma and its clinical significance

目的 :研究胰腺癌组织中c-erbB - 2、nm2 3-H1及增殖细胞核抗原 (PCNA)的表达与生物学行为之间的关系。方法 :用免疫组织化学方法 (SABC)对 39例胰腺导管癌c-erbB - 2 ,nm2 3-H1基因和PCNA的表达进行检测。结果 :39例胰腺导管癌中c -erbB - 2 ,nm2 3-H1基因蛋白的表达率、PCNA增殖指数分别为 46 .15 %、6 6 .6 7%、2 7.5± 16 .4;c-erbB - 2 ,nm2 3-H1与组织学分级有关 (P <0 .0 5 ) ,而与临床分期关系不大 (P >0 .0 5 ) ;PCNA增殖指数与组织学分级和临床分期有关 (P <0 .0 1) ;c -erbB - 2 ,nm2 3-H1,PCNA均与淋巴结转移呈正相关。结论 :c -erbB - 2 ,nm2 3-H1。

Objective:To investigate the expression of c-erbB-2 , nm23-H1, PCNA and the clinicobiological behavior in human pancreatic adenocarcinoma.Methods:The expression of c-erbB-2,nm23-H1 and PCNA in 39 cases of human pancreatic adenocarcinoma were investigated with immunohistochemistry of avidin-biotin complex. Results:In the 39 cases with pancreatic ductal adenocarcinoma, the positive rate of c-erbB-2 and nm23-H1 proteins was 46.15%, 66.67%, respectively. The PCNA LI was 27.54±16.42. The positive rate of c-erbB-2 and nm23-H1 were related to the histological grades (P<0.05),but not to clinical stages (P>0.05).PCNA labeling index were significantly different in histological grades and clinical stages(P<0.01). The expression of c-erbB-2,nm23 and PCNA were positively correlated with lymph node metastasis.Conclusion:The expression of c-erbB-2, nm23-H1 and PCNA are important markers reflecting the biological behaviors and prognosis of pancreatic carcinoma.