胰岛素样生长因子-Ⅰ对兔关节软骨细胞增殖及代谢的影响

EFFECT OF INSULIN-LIKE GROWTH FACTOR-I ON CULTURED ARTICULAR CHONDROCYTES OF RABBITS

目的 探讨胰岛素样生长因子 - (IGF- )对体外生长的关节软骨细胞分裂增殖及功能代谢的影响。方法 采用体外单层培养的方法 ,应用兔关节软骨细胞 ,对照组培养液为 10 %小牛血清 DMEM,实验组在培养液DMEM中加入 IGF- 使其终浓度分别为 3、10、30、10 0及 30 0 ng/ ml,作用细胞 2、4和 6天 ,检测细胞 DNA含量和基质中糖醛酸的含量。结果 IGF- 在 3～ 30 0 ng/ ml浓度范围能明显增加培养软骨细胞的 DNA及糖醛酸的含量 ,且以30～ 10 0 ng/ ml作用 4天刺激效果最明显 ,与对照组相比 ,有统计学意义 (P<0 .0 1)。结论 IGF- 对体外培养软骨细胞有刺激 ,并以剂量时间依赖性方式影响增殖及功能代谢。

Objective To investigate the influence of insulin like growth factor I (IGF I) on biological characteristics of articular chondrocytes cultured in vitro of rabbits. Methods Monolayer articular chondrocytes of 4 week old rabbits were cultured in medium with IGF I, at the concentrations of 3, 10, 30, 100, and 300ng/ml. The DNA content in cells and glucuronic acid content in matrix were detected on the 2nd, 4th, 6th days after culture. Results The DNA content in cells and the glucuronic acid content in matrix in articular chondrocytes cultured in medium with IGF I at concentrations of 3-300ng/ml were all significantly higher than those in control group ( P< 0.01), which reached the peak at the concentrations of 30-100mg/ml on the 4th day. Conclusion IGF I could obviously promote the proliferation of articular chondrocytes in vitro, and there exist time dependent and dose dependent effect.