几丁聚糖和透明质酸钠抑菌作用的研究

EXPERIMENTAL STUDY ON BACTERIOSTASIS OF CHITOSAN AND SODIUM HYALURONATE

目的 比较几丁聚糖和透明质酸钠的抑菌作用及抑菌范围。方法 对奇异变形杆菌、大肠埃希氏菌、白色念珠菌、铜绿假单胞菌及金黄色葡萄球菌进行培养。每种细菌培养 33管 ,其中 30管分为 A、B及 C组 ,按倍比稀释法在 A组加入高相对分子量 (Mr)几丁聚糖 ,B组加入低 Mr几丁聚糖 ,C组加入透明质酸钠 ;另 3管为对照组 (D组 )。37℃恒温下培养 18小时 ,观察细菌的生长情况。结果 A组最小抑菌浓度 (MIC)为 :奇异变形杆菌 0 .0 31% ,大肠埃希氏菌 0 .0 6 3% ,白色念珠菌 0 .0 6 3% ,铜绿假单胞菌 0 .0 6 3% ,金黄色葡萄球菌 0 .0 6 3% ;B组 MIC为 :奇异变形杆菌0 .12 5 % ,大肠埃希氏菌 0 .2 5 % ,白色念珠菌 0 .2 5 % ,铜绿假单胞菌 0 .2 5 % ,金黄色葡萄球菌 0 .12 5 %。 C组和 D组各管都有细菌生长。结论 透明质酸钠无抑菌作用 ;几丁聚糖有广谱抑菌作用 ,而高相对分子量几丁聚糖抑菌能力较强。

Objective To compare the effect and coverage of bacteriostasis of chitosan and sodium hyaluronate. Methods Each of the five bacteria, Proteus mirabilis, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Staphylococcus aureus, was cultivated for 33 tubes of broth culture. Leaving three tubes each group as control group, ploidy diluted concentration of high relative molecular weight chitosan, low relative molecular weight chitosan and sodium hyaluronate were added respectively in the broth culture. All the tubes were cultivated for 18 hours at 37 ℃ with homeothermia. Then the growth of bacteria was observed. Results The minimal inhibitory concentrations (MIC) of high relative molecular weight chitosan were : Proteus mirabilis 0.031%, Escherichia coli 0.063%, Candida albicans 0.063%, Pseudomonas aerugionosa 0.063%, Staphylococcus aureus 0.063%; and the MIC of low relative molecular weight chitosan were: Proteus mirabilis 0.125%, Escherichia coli 0.025%, Candida albicans 0.25%, Pseudomonas aeruginosa 0.25%, Staphylococcus aureus 0.125%; bacteria grew well in each tube of sodium hyaluronate group and control group. Conclusion The above results show that sodium hyaluronate has no bacteriostasis, while chitosan has bacteriostasis on broad spectrum and high relative molecular weight chitosan has stronger effect.