迈氏唇鞭毛虫致病性的研究

STUDY ON THE PATHOGENICITY OF CHILOMASTIX MESNILI

目的 探讨迈氏唇鞭毛虫的致病性。 方法 从腹泻患儿新鲜粪便内 ,浓集、纯化迈氏唇鞭毛虫包囊。用生理盐水制成包囊悬液 ,感染小鼠 ,15～ 30 d分期剖杀 ,取鼠盲肠腔内粪便 ,生理盐水涂片后镜检 ,同时取盲肠制作病理切片 ;另粪检因腹泻及 (或 )腹痛来就诊的患者。 结果 实验鼠包囊及 (或 )滋养体检出率为 88.33% (5 3/ 6 0 )。实验动物病理表明 ,迈氏唇鞭毛虫可引起鼠肠粘膜损害 ,其损害程度与感染包囊量无明显关系。但在免疫抑制剂的作用下 ,鼠肠粘膜损害明显加重。临床病例观察表明 ,实验组检出率为 16 .6 4 % (2 0 4 / 12 2 6 ) ,与对照组迈氏唇鞭毛虫感染率和感染度比较 ,差异有高度显著性。

Objective To study the pathogenicity of Chilomastix mesnili . Methods Fresh stools from children with diarrhea were concentrated and purified for cysts of C. mesnili . The suspension liquid of cysts made of physiological saline infected mice, whose bodies were cut up by stages with 15-30 d, stools from caeca of mice were examined with physiological saline on slices and pathological sections made of caeca were also examined, at the same time. Another method is stool examination sent by the patient with diarrhea or bellyache. Results The rate of cysts and/or the trophozoites of experimental mice is 88.33%(53/60). Pathology of experiment on mice suggests that C. mesnili can cause the damage to mucous membrane of murine intestines, the extent of which is not clearly related to the amount of infected cysts, but aggravated with the function of immunosuppressant. The observation of the clinical cases shows that the rate of the experimental group is 16.64%(204/1 226), and the difference from the infection rate and the infection degree of C. mesnili is significant between the experimental group and the control group. Conclusion C. mesnili are likely to be opportunistic pathogenic protozoa.