青年与老年结直肠癌C -erbB - 2不同表达与DNA倍体的关系

Relationship between DNA ploidy and different expression of C-erbB-2 oncoprotein in young and old patients with colorectal cancer

目的 探讨青年和老年结直肠癌 (CRC)患者C -erbB - 2蛋白的表达与病理学和细胞DNA倍体的关系。方法 采用免疫组化法检测 63例青、老年CRCC -erbB - 2 ,流式细胞学分析青年和部分老年患者的DNA倍体。结果 C -erbB - 2蛋白阳性表达在 31例青年CRC中有 1 4例 (45 .2 % ) ,而 32例老年CRC中仅 4例 (1 2 .2 % ) (P <0 .0 1 ) ;癌的DNA非二倍体发生率青年组为 87.1 % (2 7/ 31 ) ,老年组为 2 8.6 % (4/ 1 4 ) (P <0 .0 0 1 )。在腺癌、高 -中分化、无局部淋巴结转移及浅度浸润的癌中 ,青年组C -erbB - 2蛋白的阳性表达和非二倍体发生率均高于老年组 (P <0 .0 5 ,P <0 .0 0 1 )。结论 青年和老年CRCC -erbB - 2蛋白表达不同与细胞DNA倍体密切相关 。

Objective To explore different of expression of the C-erbB-2 oncoprotein between young and old patients with colorectal cancer(CRC) and its relation with DNA ploidy and pathological characteristics.Methods C erbB 2 oncoprotein expression was detected in 63 cases of young and senile CRC patients using immunohistochemistry, and DNA ploidy was analyzed in all young and some old patients by flow cytometry.Results Positive expression was observed in 14 cases out of 31 young patients(45.2%),but 4(12.2%) in 32 senile patients(P<0.01). The incidence of nondiploidy was 87.1%(27/31)in young group and 28.6%(4/14)in elder group(P<0.001).The positive C-erbB-2 expression and nondiploidy rate of young patients were higher than that of senile patients in the following cancers:adenocarcinoma, well-moderate differentiated, non lymph node metastasis and low infiltrated, respectively(P<0.05, P<0.001).Conclusion The difference of C-erbB-2 expression is related to DNA ploity of cell of CRC between young and senile patients,which conduces to understand higher malignance of young patients.