CRKL基因对K562细胞的作用研究

The role of CRKL gene in K 562 cell lines

目的 :通过研究CRKL基因反义寡核苷酸 (ASODN)对K 5 6 2细胞增殖及凋亡的作用 ,探讨CRKL基因对慢性粒细胞性白血病 (CML)发病的影响。方法 :应用CRKL基因反义寡核苷酸作用于K 5 6 2细胞 ,通过3 H TdR掺入、流式细胞术、Rt PCR等方法观察K 5 6 2细胞形态学、细胞周期、细胞动力学及基因表达等变化。结果 :CRKL基因ASODN对K 5 6 2细胞的增殖有抑制作用 ,并可诱导K 5 6 2细胞的凋亡。结论 :CRKL基因在CML的发病中可能有重要作用。

Objective:To investigate the effects of CRKL gene antisense-oligonucleotides(ASODN) on cell proliferation and apoptosis in K 562 cell line and thus assess the effect of CRKL gene in the pathogenesis of CML.Method: K 562 cells were cultivated with CRKL oligos which were mediated by cationin liposome. The cell viability, proliferation, apoptosis, cell cycle and gene expression were examined by 3 H-TdR doping , FCM and RT-PCR.Result: CRKL-ASDON could inhibit the proliferation of K 562 cells and induce apoptosis of K 562 cells.Conclusion: CRKL gene may play a key role in the pathogenesis in Ph-positive CML.