细胞因子对白血病抑制因子和IL-6在骨髓基质细胞表达的调节

The modulation of cytokines on the expression of leukemia inhibitory factor and Interleukin-6 of the bone marrow stromal cells

目的 :研究白细胞介素 1(IL 1)、TNF α、白细胞介素 4 (IL 4 )对骨髓基质细胞白血病抑制因子 (LIF)和白细胞介素 6 (IL 6 )表达的调节。方法 :用酶联免疫吸附试验 (ELISA)测定各细胞因子作用下的LIF与IL 6水平 ,用逆转录 聚合酶链反应 (PT PCR)测定各细胞因子作用下的LIF与IL 6转逆子水平。结果 :在IL 1、TNF α作用下LIF与IL 6水平明显升高 (P <0 .0 5 ) ,IL 4作用下IL 6水平明显升高 (P <0 .0 5 ) ,而LIF水平却明显降低 (P <0 .0 5 )。结论 :IL 1、TNF α有上调骨髓基质细胞LIF与IL 6表达 ,IL 4能上调骨髓基质细胞IL 6表达 ,却下调LIF的表达。

Objective:To study the effects of interleukin(IL)-1,TNF-α,IL-4 on the expression of Leukemia inhibitory factor (LIF),IL-6 of the bone marrow stromal cells. Method:LIF?IL-6 level were measured by an enzyme-Linked immunosorbent assays(ELISA), LIF?IL-6 mRNA were measured by reverse transcription-polymerase chain reaction(PT-PCR).Result:LIF were significantly higher in groups of IL-1,TNF-α treated (P< 0.05),and decreased significantly in the group of IL-4 treated (P< 0.05) than that in the group of control. IL-6 were significantly higher in groups IL-1,IL-4,TNF-αtreated (P< 0.05) than that in the control group.Conclusion:Stimulated by IL-1,TNF-α, the expression of IL-6 and LIF on the bone marrow stromal cells increased.Stimulated by IL-4, the expression of IL-6 on the bone marrow stromal cells increased, and LIF was inhibited.