加味四逆散对慢性心理应激大鼠的神经内分泌和行为学的调整作用

Regulatory Effect of Jiawei Sini San on Neuroendocrine and Behavior of Rats Under Chronic Psychological Stress

[目的]观察慢性心理应激对大鼠神经内分泌和行为学的影响以及加味四逆散(由柴胡、白芍、枳壳、枸杞子、桅子、干地黄、石决明等中药组成)的调治作用。[方法]大鼠随机分为3组,即正常对照组、模型组、加味四逆散组。采用放射免疫方法检测大鼠血浆皮质醇和促肾上腺皮质激素含量;以旷场实验和鼠尾悬挂实验研究慢性心理应激对大鼠行为学的影响。[结果]慢性心理应激大鼠下丘脑-垂体-肾上腺皮质轴(hypothalamus-pitutary-adrenal gland axis,HPAA)功能亢进,并出现抑郁样行为。加味四逆散可抑制慢性心理应激大鼠HPAA的兴奋性,改善慢性心理应激大鼠的抑郁表现。[结论]慢性心理应激可使大鼠神经内分泌及行为发生异常改变,而加味四逆散对此具有一定的调治作用。

[Objective] To observe the influence of chronic psychological stress on neuroendocrine and behavior of rats and to explone the regulatory effect of Jiawei Sini San (JSS). [Methods] Rats were randomly allocated to three groups: normal control group (Group A), model group (Group B) and JSS group (Group C). Plasma corticosterone and adreno-corticotrophic hormone (ACTH) were detected by radioimmunoassay. Open-field test and tail-hanging test were carried out to observe the changes of behavior caused by chronic psychological stress. [Results] Chronic psychological stress enhanced the excitability of hypothalamus-pitutary-adrenal gland axis (HPAA) and induced the occurrence of depressive behavior. [Conclusion] Chronic psychological stress can result in abnormal changes of neuroendocrine function and behavior and JSS exerts an regulatory effect on the above changes.