摘要
目的 研究克拉霉素对血管生成的影响并在此基础上观察其抗肿瘤转移的作用 ,为其临床应用提供实验依据 .方法 采用 b- FGF诱导小鼠腹壁 Matrigel种植体方法进行血管生成实验 ,比较克拉霉素处理组和对照组 Matrigel种植体内血红蛋白含量和微血管面积的差异 .采用 L ewis肺癌C5 7BL /6小鼠模型 ,观察比较克拉霉素对肿瘤组织内微血管密度、移植瘤体积、肺脏转移和实验小鼠存活时间的影响 .结果 克拉霉素剂量大于或等于 4 0 mg· kg- 1· d- 1时 ,Matrigel种植体中血红蛋白含量和微血管面积开始明显减少 ,并具有剂量 -效应关系 .克拉霉素能够明显降低肿瘤组织内的微血管密度 [(2 7± 10 ) vs (14± 7) ,P=0 .0 4 2 ],肺脏转移瘤数量 [(10± 5 ) vs (17± 9) ,P=0 .0 39]并明显延长荷瘤小鼠的存活时间 [(2 7± 10 ) d vs(19± 8) d,P=0 .0 2 2 ].结论 克拉霉素对 b- FGF诱导和肿瘤组织内的血管生成均具有明显抑制作用 ,能够有效减少荷瘤小鼠的肿瘤转移并延长存活时间 。
AIM To study the inhibitory effect of clarithromycin on angiogenesis and tumor metastasis in mice. METHODS Using quantitative analysis to compare hemoglobin and microvascular areas in Matrigel implant between the study group and the control group. The quantitation of microvessel density in tumor, occurrence of lung metastasis, tumor volume and mean survival time were analyzed in Lewis tumor bearing C57BL/6mice. RESULTS Quantitative analysis of hemoglobin and microvascular areas in Matrigel implant indicated that clarithromycin can inhibit angiogenesis at high concentration. Clarithromycin effectively reduced the quantitation of microvessel density in tumor, the number of tumor nodule in the lung and increased the mean survival time of tumor bearing mice. CONCLUSION Clarithromycin has inhibitory effect on angiogenesis and tumor metastasis and might have therapeutic value in tumor treatment.
出处
《第四军医大学学报》
北大核心
2002年第13期1214-1217,共4页
Journal of the Fourth Military Medical University
基金
陕西省自然科学基金资助项目 (2 0 0 1SM48)
