糖尿病患者T_3核受体各亚型mRNA与中医辩证关系

Association of thyroid hormone receptor mRNA in peripheral leukocytes in DM with the syndrome differentiation of TCM

目的 了解糖尿病患者外周血白细胞 T3核受体与糖尿病 (无 /有慢性并发症 )中医辩证的关系 .方法 根据是否并发慢性并发症将 2 0例糖尿病患者分为无慢性并发症组(A组、气阴两虚型 )、慢性并发症组 (B组、阴阳两虚型 ) ,应用半定量 RT- PCR法 ,观察 T3核受体各亚型 m RNA变化 .结果 B组比 A组糖尿病患者外周血白细胞中 T3Rα1 ,T3Rα2表达增强 (P<0 .0 5 ) ,T3Rβ1 ,T3Rβ2 m RNA变化不大 (P>0 .0 5 ) .结论 在外周血白细胞中 ,T3R m RNA各亚型表达有助于临床对中医气阴两虚。

AIM To study the association of thyroid hormone receptor mRNA inperipheral ldukocytes in diabetes mellieus (DM) with the syndrome differentiation of TCM. METHODS By using semi quantitative RT PCR method, we examined T 3R mRNA levels in peripheral leukocytes of DM(A,B). RESULTS T 3Rα1, T 3Rα2 mRNA levels were all increased in DM. But B was more than A ( P <0.05). CONCLUSION Measurement of the distribution of T 3R mRNA is helpful to the syndrome differentiation of TCM.