锌金属硫蛋白对镉中毒小鼠肾损伤的修复作用

The renovating effect of zinc-metallothionein to cadmium-induced oxidative damage in kidney

目的 研究锌金属硫蛋白 (Zn MT)对镉中毒小鼠肾损伤的修复作用。方法 以昆明种小鼠作为研究对象 ,染镉 1 4d建立亚急性镉中毒模型 ,随后经口给予Zn MT。收集 2 4h尿液 ,测定尿N 乙酰 β D 氨基葡萄糖苷酶 (NAG酶 )活性作为衡量肾脏损伤程度的一项指标 ,同时电镜观察肾组织形态学变化 ;测定并分析肾组织上清液中脂质过氧化代谢产物—丙二醛 (MDA)水平、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化酶 (GSH Px)活性。结果 Zn MT可明显降低肾组织中MDA水平 ,使肾组织中SOD、GSH Px 活力有一定程度的恢复 ,此时尿NAG酶活性降低表明肾损伤程度减轻 ,且上述作用呈明显的剂量 反应关系 ;电镜下观察到给予Zn MT后肾组织形态学病变有所减轻。结论 Zn MT可对镉中毒小鼠肾组织脂质过氧化损伤起到一定的修复作用。

Objective To study the renovating effect of zinc metallothionein to cadmium induced oxdative damage in kidney Methods Kunming mice were used to establish the cadmium poisoning models, then treated with zinc metallothionein(Zn MT).Then indexes were assayed, including the contents of malony diadehyde(MDA),the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH P x)in kidney, the activities of NAG in urine and the ultrastructure of kidney were studied under transmission electron microscope Results In animals which administated Zn MT, the contents of MDA in kidney decreased and the activities of antiosidase (SOD, GSH P x)renovated, and the activities of NAG in urine decreased the pathomorphologic damages of kidney induced by cadmium were renovated to some extent after administrating Zn MT Conclusion Zn MT may have the role of renovating effect on cadmium induced oxidative damage in kidney