猪链球菌病氢氧化铝二联灭活疫苗猪体血清抗体消长规律

Rising and Falling Rule of Serum Antibodies in Pigs vaccinated with Swine Streptococcosis Inactivated Bi-combined Vaccine

应用琼扩法 (AGP)、间接ELISA法检测猪链球菌病氢氧化铝二联灭活疫苗免疫猪的抗体水平。结果表明 ,免疫后 4天开始产生抗体 ,7天时免疫猪 10 0 %抗体阳转 ,15～ 30天抗体效价达高峰。免疫后 35天进行二免 ,二免后 5天抗体效价均显著升高 ,6个月后血清抗体仍维持在 1∶16 0 0。母猪二免可使仔猪获得高水平且持勺的抗体 ,35日龄其母源抗体仍保持在 1∶80 0。通过免疫抗体与攻毒保护相关性测试 ,抗体效价在 1∶80 0 (含 1∶80 0 )以上能抵抗 1× 10 7CFU马腺疫链球菌兽疫亚种和 1×10 8CFU猪链球菌

The serum antibody titer were examed with AGP and ELISA methods in pigs vaccinated with the inactivated bi_combined vaccine of swine Streptococcosis.The results showed that antibodies appeared 4 days after vaccination,turn_positivation rate of antibodies was 100% after 7 days,anibody titer reached summit during 15 to 30 days.Second vaccination was done at 35th day.Antibody titer began to rise obviously after 5 days of second vaccination,the titer was still maintained at 1∶1 600 after 6 months vaccination.Maternal antibody titer of sucking pigs produced by sow vaccinated two times was higher and lasting.The test also showed that the pigs could resist 1×10 7 CFU Streptococcus equi subsp zooe pidemicus  and 1×10 8CFU Suis type 2 attack respectively when antibody titer was not less than 1∶800.