应用组织培养技术进行三倍体毛白杨快繁的研究

Study on Quick Propagation of Triploid Chinese White Poplar Trees by Using the Technology of Tissue Culturing

应用植物组织培养技术对三倍体毛白杨快速繁殖进行了研究 ,结果表明 ,适宜三倍体毛白杨茎段芽增殖的培养基为MS +BA 0 5mg/L +NAA 0 0 1～ 0 0 2mg/L ,适宜叶切块产生不定芽的最佳培养基为MS +BA 0 15～ 0 5mg/L+NAA 0 0 2～ 0 0 5mg/L +IAA 0～ 0 2 5mg/L ,在此培养基上 ,可建立良好的快速繁殖体系。适宜芽生根的培养基为MS +IBA 0 2mg/L ,生根率在 10 0 %。

The quick propagation of triploid white Chinese poplar has been studied by applying the technology of plant tissue culturing. It is shown by the results that the culture medium which suits bud proliferation at stem section of triploid white Chinese poplar is MS+BA 0.5 mg/L+NAA 0.01～0.02 mg/L and the best culture medium which suits leave slicing for producing adventitious bud is MS+BA 0.15～0.5 mg/L+NAA 0.02～0.05 mg/L+IAA 0～0.25 mg/L. The better quick propagation system can be established based on the culture medium. The culture medium that suits bud root is MS+IBA 0.2 mg/L and root rate is 100%.