釉基质蛋白对成骨细胞矿化及粘附能力的影响

The Studies on Osteoblasts' Mineralization and Attachment Affected by Enamel Matrix Proteins

目的观察釉基质蛋白(EMPs)对成骨细胞矿化和粘附能力的影响,进一步探讨EMPs促进牙槽骨再生的机理。方法在培养液中加入不同浓度的EMPs,体外培养MC3T3-E1成骨细胞,用Von-Kossa染色法检测矿化结节的形成情况,粘附细胞计数法检测细胞的粘附能力。结果EMPs能明显促进MC3T3-E1成骨细胞的矿化能力(P<0.01),但对细胞的粘附能力无明显影响(P>0.05)。结论EMPs能提高成骨细胞的成骨能力,对成骨细胞在根面的定植无影响,提示这可能是其促进牙槽骨再生的一个重要原因。

Objective Investigate the effects of enamel matrix proteins(EMPs) on the mineralization and attachment of osteoblasts,to explore the possible mechanism of alveolar bone regeneration induced by enamel matrix proteins(EMPs).Methods MC3T3-E1 osteoblasts were cultured in vitro and EMPs was added to medium in several concentrations. Cell's mineralizing ability was detected by Von-Kossa stain and attachment ability was detected by counting cells number. Results The results manifested that EMPs promote more mineral nodules formation (P<0.01). But MC3T3-E1 cells' attachment ability was not improved (P>0.05). Conclusions EMPs enhanced the mineralizing function of osteoblasts, had no effects on implanting on root surface of osteoblasts, which may play an important role in the regeneration of alveolar bone.