抗变形链球菌鸡卵黄抗体对变形链球菌合成葡聚糖影响的实验研究

The Inhibitory Effect of Anti-Streptococcus Mutans Immunoglobulin of Yolk on Glucan Synthesis of Streptococcus Mutans

目的研究抗变形链球菌鸡卵黄抗体(IgY)对变形链球菌合成葡聚糖的影响。方法用蒽酮法测定各浓度组IgY使用后,定量菌在单位时间内合成葡聚糖的量。结果IgY抗体对血清c型及血清g型变形链球菌合成胞外多糖均有影响,各浓度组IgY对合成胞外多糖的影响力有随着浓度升高而增强的趋势。1∶16浓度组起有效,1∶2浓度组的抑制作用最强(P<0.05)。结论IgY可以减少变链菌合成葡聚糖。

Objectives To observe the inhibitory effect of anti-Streptococcus mutans Hen-egg-yolk (IgY) on glucan synthesis of Streptococcus mutans. Methods Determine the glucan with anthrone method after using the different titer of IgY on S.mutans and S.sobrinus. Results The inhibitory effect of antibody on S.mutans and S.sobrinus synthesis glucan had a similar tendency. The effect of inhibit glucan synthesis was influenced by the titer of antibody. High titer with high inhibition. Begin with the group of 1∶16 titer, the group of 1∶2 titer showed the highest inhibition（P<0.05）. Conclusion The antibody can reduce synthesis of glucan from S.mutans and S.sobrinus.