乳癌组织中GST-Pi,ToPoII和PgP耐药基因的表达

Significance in expression of GST-Pi,ToPoII and PgP resistance drug gene in breast carcinoma

目的 探讨乳癌组织中GST Pi ,ToPoII和PgP耐药基因的表达及其生物学意义。 方法 采用即用型免疫组化微波加热法 ,检测 5 2例乳癌GST Pi ,ToPoII和PgP耐药基因。结果 (1) 5 2例乳癌中的GST Pi ,ToPoII和PgP的阳性表达率分别为 5 0 .0 % (2 6/ 5 2 ) ,17.3 % (9/ 5 2 )和 11.5 % (6/ 5 2 ) ;(2 )浸润性导管癌对三种耐药基因均呈不同程度的阳性表达 ,较其它组织学类型的乳癌的耐药基因更为广泛。 (3 )乳癌中的GST Pi ,ToPoII和PgP阳性表达大多与年龄无明显关系 ,但 40～ 49岁和 60～ 69岁年龄组的三种耐药基因阳性表达较其他年龄组为高。结论 (1)部分乳癌患者的体内存在多向耐药基因 ,或癌细胞内 (原发 )存在GST Pi ,ToPoII和PgP ,对抗癌药物已产生耐药性。 (2 )对乳癌患者在化疗前进行耐药基因检测 ,有助于联合用药的合理选择、提高疗效及延长患者生存期。

Objective To explore the expression and biological significance of resistance gene of GST Pi,ToPoII and PgP in breast carcinoma. Method To test and analyse the resistance gene for GST Pi,ToPoII and PgP of 52 cases of breast carcinoma was made by using instant microwave heating immunohistochemistry.Results (1) Positive expression rates of GST Pi,ToPoII and PgP in 52 cases of breast carcinoma were 50.0% (26/52), 17.3% (9/52) and 11.5%(6/52),respectively. (2) Invasive ductal carcinoma had positive expression of three resistance genes at different levels,and the resistance genes in invasive ductal carcinoma were more widely than those in other types breast cancer. (3) There was no obvious relationship between positive expression of GST Pi,ToPoII and PgP in breast carcinoma with patients' age. However,the positive expression of three resistance genes in patients aged 40 to 49 and aged 60 to 69 were higher than other ages.Conclusions (1) The multidrug resistance genes existing in some patients of breast carcinoma or the primary cancer cell has resistance to GST Pi,ToPoII and PgP antineoplastics. (2) Before chemotherapy to patients with breast carcinoma, the test of resistance gene should be done, which will help doctors select a resonable method of combination of drugs so as to improve curative effect and to prolong patients' life.