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抗乙酰胆碱受体单链抗体在大肠杆菌中的表达 被引量：2

Expression of single chain variable fragment of antibody against acetylcholine receptor in E coli
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摘要 [目的 ]使构建的抗人乙酰胆碱受体单链抗体基因在大肠杆菌中表达 .[方法 ]将含有单链抗体基因的载体 pHEN2 (含有 pelB引导肽和c myc)转化大肠杆菌HB 2 15 1,在细菌外周质中表达单链抗体 .应用鼠抗c myc单链抗体的斑点杂交试验检查细菌外周质裂解物中单链抗体的表达 .[结果 ]单链抗体表达在细菌外周质中 ,培养液上清及对照大肠杆菌外周质和培养液上清均未发现单链抗体 .[结论 ]已成功地在大肠杆菌中表达了抗人乙酰胆碱受体单链抗体 . OBJECTIVE?To express the genes of a single chain variable fragment of antibody against human acetylcholine receptor in E coli.?METHODS?The single chain variable fragment genes in the vector pHEN2 bearing a pelB leader and a c myc tag were transformed into E coli HB?2151, and expressed in bacterial periplasm. The expression was detected by dot blot assay using a mouse anti c myc monoclonal antibody.? RESULTS ?The single chain variable freagment was found in periplasmic fraction, however no expression was observed in culture medium, or in periplasmic fraction and culture medium of control E coli HB?2151.? CONCLUSION ?The genes of a single chain variable fragment of antibody against human acetylcholine receptor have been successfully expressed in E coli.
作者 杨康鹃 孟繁平 M.Stassen M.de Baets
机构地区 延边大学医学院生物学教研室 延边大学医学院微生物学免疫学教研室 荷兰马斯特里赫特大学医学院神经学系
出处 《延边大学医学学报》 CAS 2002年第2期79-82,共4页 Journal of Medical Science Yanbian University
基金 国家自然科学基金 (3996 0 0 72 ) 教育部高等学校骨干教师资助计划基金
关键词 抗乙酰胆碱受体 单链抗体 大肠杆菌 基因表达 重症肌无力 myasthenia gravis single chain variable fragment receptors,cholinergic escherichia coli
分类号 R378.21 [医药卫生—病原生物学] R392.1 [医药卫生—免疫学]
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  • 8杨康鹃,孟繁平,YGraus,MdeBaets.重组质粒对大肠杆菌 DH5_α 的转化[J].延边大学医学学报,1997,20(2):69-70. 被引量：3

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同被引文献19

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延边大学医学学报
2002年 第2期

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