鳞片愈伤组织繁殖的水仙在旱作条件下的性状

THE CHARACTERS OF PLANTS PROPAGATED FROM NARCISSUS TAZETTA SCALE CALLUS CULTURES UNDER DRY FARMING

水仙是多年生草本植物,常规采用分球繁殖,繁殖率低。利用组织培养技术,通过愈伤组织增殖可以迅速扩大形成小植株的基础,但从愈伤组织产生的植株常发生变异(Hussey,G.1978,Science Progress)。本文报道,由鳞片愈伤组织培养物所繁殖的水仙在旱作条件下的性状。 材 料 与 方 法 材料 水仙(Narcissus tazetta var.chinensis Roem.)鳞片切块(带鳞茎盘)在附加BA 1mg/1和 2,4-D 0.1mg/1的MS培养基上诱导产生愈伤组织,并在相同的培养基上继代培养。将12和13代愈伤组织转至含NAA 0.1mg/1的MS培养基上,分化出不定芽,最终不定芽枯萎并在其基部形成休眠的小鳞茎。在水仙自然播种季节前约半个月,将小鳞茎转至含NAA 0.03mg/1的1/2 MS培养基上,抽叶生根后,移植田间,次年5月采挖,选取球径为1.8—2.2cm的小鳞茎50个作试验材料。

The bulblets formed the implanting plantlets from Narcissus tazetta scale callus cultures are taken for the experimental material and the bul-blets arising from natural vegetative increases are taken for the control. The experimental ones must be planted continuous for 4 - 5 years to bloom. Their capacity of daughter bulb formation and morphological characters are not significantly different to them of the control.