转化生长因子-β_2对牛眼小梁细胞外基质合成的影响

Effect of transforming growth factor-β_2 on extracellular matrix synthesis in bovine trabecular meshwork cells

目的 观察人眼房水中转化生长因子 β2 (transforminggrowthfactor β2 ,TGF β2 )对体外培养牛眼小梁细胞外基质合成的影响。方法 将体外培养的第 3～ 5代牛眼小梁细胞分为对照组和实验组 ,实验各组分别经 0 32× 10 3 ng/L、1 0 0× 10 3 ng/L、3 2 0× 10 3 ng/LTGF β2 作用 4 8h后 ,分别应用3 H 脯氨酸掺入及液体闪烁测量技术、酶联免疫吸附法和放射免疫技术检测小梁细胞胶原 (collagen ,CA)、纤维连接蛋白 (fibronectin ,FN)及透明质酸 (hyaluronicacid ,HA)的合成。结果 与对照组相比 ,不同浓度的TGF β2 均可促进牛眼小梁细胞CA的合成。 0 32× 10 3 ng/L、1 0 0× 10 3 ng/LTGF β2 组与对照组比较差异有显著意义 (q′=3 85、4 38,P <0 0 5 ) ,3 2 0× 10 3 ng/LTGF β2 组与对照组比较差异有非常显著意义 (q′ =11 4 0 ,P <0 0 1) ,3 H 脯氨酸掺入量呈浓度依赖性增高 ;1 0 0× 10 3 ng/L、3 2 0× 10 3ng/LTGF β2 组促进牛眼小梁细胞合成FN ,与对照组比较差异有显著意义 (q′=3 6 3,P <0 0 5 )和非常显著意义 (q′ =2 0 10 ,P <0 0 1) ;1 0 0× 10 3 ng/L、3 2 0× 10 3 ng/LTGF β2 组均抑制牛眼小梁细胞合成HA ,与对照组比较差异有非常显著意义 (q′=15 15、16 92 ,P <0 0

Objective To investigate the effect of transforming growth factor β 2 (TGF β 2) in human aqueous humor on extracellular matrix synthesis in cultured bovine trabecular meshwork cells. Methods Cultured 3 5 passage bovine trabecular meshwork cells were divided into control group and experimental group. After treated with 0 ng/L (control), 0 32×10 3 ng/L, 1 00×10 3 ng/L, 3 20×10 3 ng/L TGF β 2 for 48 h, collagen, fibronectin and hyaluronic acid synthesis in bovine trabecular meshwork cells were examined respectively by 3H proline incorportion and liquid scintillation technique, enzyme linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Results In comparison with the control group, TGF β 2 significantly promoted the synthesis of collagen at 0 32×10 3 ng/L ( P <0 05), 1 00×10 3 ng/L ( P <0 01), 3 20×10 3 ng/L ( P <0 01), 3H proline incorporation increased in a concentration dependent manner; and 1 00×10 3 ng/L ( P <0 05), 3 20×10 3 ng/L ( P <0 01) TGF β 2 significantly promoted the synthesis of fibronectin in cultured bovine trabecular meshwork cells. 1 00×10 3 ng/L ( P <0 01) and 3 20×10 3 ng/L ( P <0 01) TGF β 2 significantly inhibited the synthesis of hyaluronic acid in the cultured cells. Conclusion The data suggest that TGF β 2 play important roles in extracellular matrix age related changes of normal trabecular meshwork and abnormal deposition of the extracellular matrix in the trabecular meshwork, especially the juxtacanalicular tissue of primary open angle glaucoma patients.