粘质沙雷菌苗膀胱灌注前后肿瘤组织端粒酶活性的变化

Telomerase activity in transitional cell carcinoma after intravesical instillations of Serratia Marcescens

目的 了解粘质沙雷菌苗膀胱灌注前后肿瘤组织端粒酶活性的变化并探讨其意义。 方法 采用改良端粒重复序列扩增 (TRAP) 银染方法检测 2 0例膀胱移行上皮细胞癌肿瘤组织灌注前后的端粒酶活性。 结果 灌注后肿瘤组织端粒酶阳性率 5 0 % (10 / 2 0 )明显低于灌注前的 90 %(18/ 2 0 ) ,P <0 .0 5。用药前“ -”、“ +”、“ ++”、“ +++”患者分别为 2例、2例、10例、6例 ,用药后分别为 10例、6例、2例、2例 ,P <0 .0 1。 结论 粘质沙雷菌苗膀胱灌注可明显降低肿瘤组织的端粒酶活性 ,可能对肿瘤细胞的生长具有抑制作用。

Objective To study changes of telomerase activity in transitional cell carcinoma after intravesical instillation of Serratia Marcescens . Methods TRAP-PCR and TRAP-sliver staining were used to detect telomerase activity in 20 patients with TCC after intravesical instillation of Serratia Marcescens . Results Positive rate of telomerase activity detection in TCC patients after intravesical instillation of Serratia Marcescens was 50%(10/20),significantly lowed than before the intravesical instillation (90%,18/20), P <0.05. The number of patients with '-', '+', '++', and'+++'telomerase activity before intravesical instillation were 2, 2, 10, and 6 respectively while after the intravesical instillation these turned to be 10, 6, 2, and 2 respectively. The difference was also statistically significant ( P <0.01). Conclusions The use of intravesical instillation of Serratia Marcescens can inhibit effectively the telomerase activity in TCC, and thereby would inhibit the tumor cell growth.