“益肝浓缩煎剂”对实验性大鼠肝纤维化防治作用的研究

EFFECTS OF PREVENTION AND TREATMENT OF CONCENTRATED YIGAN DECOCTION ON EXPERIMENTAL LIVER FIBROSIS IN RATS

目的研究“益肝浓缩煎剂”对四氯化碳大鼠肝纤维化预防与治疗作用。方法以CCl4 诱导大鼠肝纤维化模型 ,注射CCl4 前 2周及造模结束后分别予“益肝浓缩煎剂”进行防治 ,各持续 10周 ;测定肝组织羟脯氨酸(hydroxyproline ,Hyp)含量 ,光镜、电镜观察肝脏病理形态变化。 结果“益肝浓缩煎剂”预防、治疗组Hyp显著降低(P <0 .0 1) ,胶原沉积及脂肪变性明显减轻 (P <0 .0 5 ) ,电镜显示“益肝浓缩煎剂”预防组肝细胞内脂滴明显减少 ,细胞器接近正常 ,狄氏间隙内仅见少量胶原纤维增生。结论“益肝浓缩煎剂”对大鼠肝纤维化具有明确防治作用。

ObjectiveTo study the preventive and therapeutic effects of Concentrated Yigan Decoction on liver fibrosis in rats induced by carbon tetrachloride(CCl 4). MethodsLiver fibrosis in rats was induced by CCl 4 and Concentrated Yigan Decoction was given 2 weeks before the beginning of CCl 4 injection(preventive group)or after model establishment (therapeutic group) respectively. The administration of the decoction lasted for 10 weeks. Liver hydroxyproline (Hyp) level was determined and histopathological changes were observed by light microscopy and electromicroscopy.ResultsIn preventive and therapeutic groups of Concentrated Yigan Decoction, Hyp and degrees of liver fibrosis and fatty degeneration were markedly reduced respectively compared with those of control groups (P< 0.05 ).Ultrastructurally,lipid droplets in hepatocytes were markedly decreased, cell organells were restored to normal generally and few collagenous fibers were seen in Disse space in the rats of preventive group.ConclusionConcentrated Yigan Decoction had remarkably preventive and therapeutic effects on liver fibrosis of rats.