小鼠血浆中马钱素的高效液相色谱测定法及药代动力学

RP-HPLC DETERMINATION OF LOGANIN IN MOUSE PLASMA AND ITS PHARMACOKINETICS

目的 建立测定小鼠血浆中马钱素浓度的高效液相色谱法 ,并研究马钱素在小鼠体内的药代动力学。方法 色谱柱为C1 8柱 ,流动相甲醇 水 (30∶70 ) ,流速为 0 8mL·min- 1 ,检测波长 2 40nm ;血浆样品用固相萃取法预处理。结果 线性范围 0 0 1～ 5 0 0 μg·mL- 1 。日内RSD <10 % ,日间RSD <15 % ,回收率 86 0 %～ 91 5 % ,最低定量浓度为10ng·mL- 1 。ig给药 0 5h后血浆药物浓度达峰值 ,Cmax 为 6 8μg·mL- 1 ,T1 2 α为 2 6 1min ,T1 2 β为 2 9 0 1min。 结论该方法灵敏度高 ,操作方便 ,适用于马钱素的药代动力学研究 ;本品口服吸收快消除也快。

AIM To develop a method for determination of loganin in mouse plasma by using high-performance liquid chromatography. The method was employed to study pharmacokinetics of loganin. METHODS An RP-C 18 was used as the stationary phase.The mobile phase consisted of methanol-water (30∶70), at the flow-rate of 0.8 mL·min -1 . The UV absorbance detector was set at 240 nm. Plasma samples were treated with solid phase extraction. RESULTS The recovery of loganin in mouse plasma was 86.0%～91.5%. The calibration curve in plasma was linear over the range of 0.01～5.00 μg·mL -1 . The limit of quantitation was 10 ng·mL -1 . The RSDs of intra-day and inter-day (n=5) were less than 15%. The pharmacokinetic parameters were C max =6.8 μg·mL -1 , T max =30 min, T 1/2 α=26.1 min, T 1/2 β=29.01 min. CONCLUSION The method is accurate, sensitive and suitable for pharmcokinetic study of loganin. The absorption and elimination of loganin were rapid after ig in mice.