碱性磷酸酶活性与退行性心脏瓣膜钙化关系的研究

RELATIONSHIP BETWEEN THE ACTIVITY OF ALKALINE PHOSPHATES AND DEGENERATIVE CARDIAC VALVULAR CALCIFICATION

采用免疫组织化学染色及生化分析方法测定不同钙化程度心脏瓣膜中碱性磷酸酶 (ALP)的生物活性 ,探讨ALP与退行性心脏瓣膜钙化的关系。结果发现 ,心脏瓣膜的纤维细胞具有产生ALP的功能 ,钙化灶周围基质中ALP表达较高 ;光镜下未见钙化及严重钙化的瓣膜中ALP生物活性比较低 ,Ⅰ、Ⅱ级钙化的瓣膜中ALP生物活性明显高于前者。本研究结果表明 :瓣膜中的纤维细胞可产生ALP ,其ALP的生物活性主要取决于瓣膜中退变细胞释放基质小泡过程的活跃程度 ,即与钙化过程的活跃程度相关。

To explore the association between alkaline phosphatase (ALP) and degenerative cardiac valvular calcification, the activity of ALP and the grades 0～Ⅳ degenerative calcified valves were determined by biochemical and immunocytochemical methods. ALP positive substance was present in the matrix around the foci of calcification and in the cytoplasm of fibroblasts. The bioactivity of ALP was much higher in grades Ⅰ and Ⅱ calcified valves than that in grades 0 and Ⅲ～Ⅳ calcified valves. The results indicate that the level of ALP bioactivity is predominantly dependent on the process of extruding matrix vesicles from senescent cells in the valves.