摘要
为探究获得大量高纯度雪旺细胞的方法 ,取生后 1～ 3天SD乳鼠的坐骨神经 ,采用改进后的植块反复移植法进行细胞培养。同时通过低浓度胰酶快速消化法、差速贴壁法和加用细胞毒药物等去除成纤维细胞 ,纯化雪旺细胞。最后用抗S 10 0蛋白酶联免疫细胞化学染色法进行细胞鉴定。结果发现 ,经植块反复移植和多种方法结合纯化后 ,雪旺细胞的纯度可达 95 %以上 ,并具有良好的活性。故认为 ,此方法简便易行 。
The aim was to look for the method for getting highly purified population and large amount of Schwann cells from the newborn rats. Sciatic nerves from 1～3 days old SD rats were taken out . The Schwann cells were cultured and purified by modified repeated explanation, and fibroblastic cells were removed by rapid trysinization , differential adhesion and anti mitosis methods. The Schwann cells were identified by indirect immunocytochemistry with mouse anti S 100 protein McAb. The result showed that the purity of Schwann cells was more than 95%, and the cells were in a good state. It is considered that the methods are simple and convenient , and a great number of highly purified Schwann cells can be acquired by them.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第7期610-611,I003,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助课题 (编号 39870 793)
