不同剂量^(60)Coγ射线对玉米幼苗生长和过氧化物酶同工酶的影响

Effects of ^(60)Coγ-Ray on Growth of Maize Seedlings and Peroxidase Isozymes

用5—30KR剂量^(60)COγ射线照射玉米种子,对幼苗生长的抑制效应随照射剂量的增加而明显增强.过氧化物酶同工酶谱测定表明,经不同剂量^(80)COγ射线照射后,叶片增加1条负极向酶带;同时在高剂量(25—30KR)照射下,有3条酶带活性明显增强.叶片正极向同工酶谱,在30KR高剂量照射后也诱导产生2条酶带.经5—25KR剂量照射后,胚轴增加1条负极向酶带,而在30KR时该酶带又消失;同时,在15—30KR下随照射剂量的增加,有1条酶带活性明显减弱.上述结果表明,经γ射线照射后,随着幼苗外部形态的变化,也发生了内部分子水平的变化,但这种变化是不完全相关的.

The seed of maize had been irradiated with different doses(0-30KR) of 80Co?-ray before sown. The experiment indicated that the inhibition on growth of maize seedlings was strengthened considerably as the, doses of irradiation increased.The peroxidase isozyrnes assay indicated as follows. Being irradiated with different doses of 60Co?-ray, a-new cathode band in zymograme of blades appeared. Meanwhile, being irradiated with large doses (25-30KR) , three bands of enzymatic-activity were strengthened obviously. And, anodic isozyme zyrnograms of blades were induced to bear two bands in zymograme while being irradiated with large dose of 30KR. Being irradiated with the doses of 5-25KR, a cathode band in zymograme of the plumular oxises increased, but it disapeared while being irradiated with the dose of 30KR. Meanwhile, as the irradiating doses increased within the range of 15-30KR, a band of enzymatic activity was clearly weakened.According to above, being irradiated with 80Co?-ray, the seedlings transformed, in both morphogenesis structure and molecular level, but it seems that there is no full relativity between them.