外源基因导入小麦引起的生化特性变化

Variation of Biochemical Properties by Introducing Exogenous DNA into Common Wheat

将外源豌豆 DNA直接导入普通小麦中 ,结果发现变异小麦的籽粒蛋白质构成及过氧化物酶 (POD)、酯酶 (EST)、超氧化物歧化酶 (SOD)同工酶谱带发生了不同类型的广泛变异 :超大穗变异株系 M1 新增加了高分子量麦谷蛋白亚基 86 ku和 80 ku组分 ,相反 ,中国春变异株系 C消失了高分子量麦谷蛋白亚基 92 ku和 82 ku组分 ,它们的中分子量蛋白区亚基带染色程度也分别产生了明显的加深和减弱变化 ;同工酶不同程度出现差异谱带、酶带缺失、酶活性增强或减弱的显著变化。表明受体发生广泛变异可能是外源基因导入、基因杂合、基因互作与外源 DNA片段“重组插入”

Exogenous pea DNA was directly introduced into common wheat. A great range of different kinds of variation , such as seed protein components, peroxidase isozyme (POD), esterase isozyme (EST) and superoxide dismutase (SOD), occurred in the progenies of receptors. The high molecular weight glutenin subunits (HMW GS) increased by 86ku and 80ku new components in Superear M 1 variant wheat, and on the other hand, in Chinese Spring C variant wheat, HMW GS components 92ku and 82ku disappeared, and the staining degree of the protein subunit bands in its MMW regions also occurred striking strengthen (intensified) and weaken separately. Isozyme also showed different isozyme bands, losing isozyme bands and increase and decrease of enzyme activity in varying degrees. It was assumed that the mechanism for variation of the receptor caused by exogenous DNA might be the comprehensive effects of the donor gene transference, gene heterology, gene interaction and the exogenous DNA fragment 'recombination insert'.