氟苯尼考静注及肌注在鸡体内药代动力学研究

The Pharmacokinetics of Florfenicol in Broiler Chickens

健康AA肉鸡 36只 ,随机分成 4组 ,以 15mg/kg和 30mg/kg两种剂量静注、肌注分别给予氟苯尼考。用高压液相色谱法测定血浆中的药物浓度 ,采用 3p97药代动力学程序软件处理药 时数据。静注药 时数据符合二室开放模型 ,主要药代动力学参数 :15mg/kg剂量组Vd(ss) 1 5 7± 0 16L/kg ,T1/2α43 96± 12 2 7min、T1/2 β16 8 18±45 2 4min、CL(s) 0 0 17± 0 0 0 30L/ (kg·min)、AUC886 40± 146 5 3(μg/ml)·min ;30mg/kg剂量组Vd(ss) 1 42±0 2 3L/kg ,T1/2α41 48± 8 6 4min、T1/2 β180 80± 74 97min、CL (s) 0 0 17± 0 0 0 2 9L/ (kg·min)、AUC176 7 15±2 6 8 2 3(μg/ml)·min。肌注药 时数据符合一室开放模型 ,主要药代动力学参数 :15mg/kg剂量组T1/2 (ka) 10 2 5±9 19min、T1/2 (ke) 15 2 41± 73 0 9min、C(max) 3 5 0± 1 13μg/ml、AUC837 88± 16 0 85 (μg/ml)·min、F94 5 3% ;30mg/kg剂量组T1/2 (ka) 11 97± 7 5 9min、T1/2 (ke) 15 2 41± 73 0 9min、C(max) 6 79± 1 38μg/ml、AUC172 5 2 9±35 7 98(μg/ml)·min、F97 6 3%。实验结果表明 :氟苯尼考在鸡体内吸收好 ,分布快 ,消除也快。静注、肌注后曲线下面积AUC与剂量呈比例关系 ,各参数无剂量依赖性。

Thirty-six broiler chickens were used to investigate the pharmacokinetics of florfenicol after single intravenous (i.v.) and intramuscular (i.m.) at two doses (15 and 30 mg/kg body weight,respectively).The plasma concentration of florfenicol was determined by HPLC.Pharmacokinetic parameters were calculated by 3p97 computer program.Concentration-time data relating to i.v.injection were analysed using two compartment open model and main parameters were as follows:15mg/kg dosage group Vd(ss)1.57±0.16L/kg,T 1/2α 43.96±12.27min?T 1/2β 168.18±45.24min?CL(s)0.017±0.0030L/(kg.min)?AUC886.40±146.53μg/ml·min;30 mg/kg dosage group Vd(ss)1.42±0.23L/kg,T 1/2α 41.48±8.64min?T 1/2β 180.80±74.97min?CL(s)0.017±0.0029L/(kg·min)?AUC 1 767.15±268.23μg/ml·min.Data relating to i.m.administration were analysed using a one compartment model and main parameters were as follows:15 mg/kg dosage T 1/2 (ka) 10.25±9.19min?T 1/2 (ke)152.41±73.09min?C(max)3.50±1.13μg/ml?AUC837.88±160.85μg/ml·min?F94.53%;30mg/kg dosage group T 1/2 (ka)11.97±7.59min?T 1/2 (ke)152.41±73.09min?C(max)6.79±1.38μg/ml?AUC 1 725.29±357.98μg/ml·min?F97.63%.Results suggested that distribution of florfenicol was rapid and wide in the broiler chickens and elimination was quick.Absorption of florfenicol following i.m.administration was almost complete.After i.v.and i.m.administration,AUCs were proportional to doses of florfenicol and all main parameters were not dose dependent.