6-苄基腺嘌呤对平菇和香菇菌丝体4种胞外酶活性的影响

The Effect of 6-Benzyl Aminopurine on the Activities of Four Extracellular Enzymes in Plurotus ostreatus and Lentinula edodes Mycelium

在马铃薯液体培养基中 ,通过添加不同剂量的 6 -苄基腺嘌呤 (6 - BA ) ,测定了平菇和香菇菌丝体 4种胞外酶活性的变化。结果表明 ,低浓度的外源 6 - BA对平菇、香菇菌丝体 4种胞外酶活性都有促进作用 ,高浓度时则逐渐抑制酶活性。其中 ,培养液浓度为 1.5 mg/ 10 0 m L时 ,它们的胞外淀粉酶 (Amy)、羧甲基纤维素酶 (CMC)、过氧化氢酶 (CAT)的活性最高 ;而它们的胞外蛋白酶的最适浓度为 0 .5 m g/ 10 0 m

Four extracellular enzyme activities in Pleurotus ostreatus and Lentinula edodes mycelium were determined by adding to different dosage additional 6 benzyl aminopurine (6 BA) in potato liquid medium. The results showed that low concentration of 6 BA promoted their activities and high concentration inhibited their activities. For Pleurotus ostreatus and Lentinula edodes, the optimum concentration of 6 BA to activate their extracellular amylase, carboxymenthel cellulase and catalase was the same which was 1 5 mg/100 mL culture solution. But, the optimum concentration for their extracellular protease was 0 5 mg/100 mL culture solution.