层孔菌产漆酶的摇瓶最适培养条件研究

STUDY ON LIQUID CONDITIONS IN SHAKING FLASKS FOR LACCASE PRODUCTION BY A WHITE ROT FUNGUS

筛选到一株高产漆酶的层孔菌W 1 ,对其液体产漆酶的培养条件进行了优化 ,确定了最适的碳源、氮源 ,并用正交试验确定了培养基中碳源和氮源的浓度。在最优化的培养条件下 ,培养 7d后 ,W 1产生的漆酶活力可以达到 7 1U/mL。采用补料的方法 ,可以得到大量的漆酶粗酶液。

A white-rot fungi Rigidoporus sp.W-1 which could produce laccase was isolated. The fermentation conditions in shaking flasks were investigated. The optimal carbon source was wheat bran and (NH 4) 2SO 4 was the optimal nitrogen source. The components of the medium were optimized by orthogonal experiment. When W-1 was cultured under the optimum conditions, the activity of laccase could get to 7.1U/mL in 7 days.A great amount of crude laccase could be obtained by adding fresh medium to the 7 days old mycelium.