摘要
采用荧光标记STR基因扫描技术对纳西族进行了STR多态性调查。 9个STR基因座在纳西族群体中 ,检出72个等位基因、16 5种基因型 ,其频率分布在 0 .0 0 5 2～ 0 .5 2 0 8和 0 .0 10 4～ 0 .30 2 1。χ2 检验表明 ,各基因座的基因型分布符合Hardy Weinberg平衡定律 (P >0 .0 5 )。统计学结果显示 ,这些遗传标记在纳西族群体中 ,H均大于 0 .6 ,平均PIC高于 0 .7,平均DP在 0 .8以上 ,EPP也都超过了 0 .5。
STR is a universal genetic marker that has changeable polymorphism and stable heredity in human genome. It is a specific DNA segment composed of 2～7 base pairs as its core sequence,and is formed through the repeated connection of the same one. Since it has the characteristics such as numerous allelic genes,highly heterozygosity and easy recognition and short PCR sagment,it is employed as an ideal DNA marker in such practical fields as human genetics and forensic medicine. In this study,we investigated the polymorphism of STR of Naci minority with STR genescan marked by fluorescece. Sevenly two alleles of 9 STR in Naci were detected with their frequency 0.0052～0.5208 and 165 genotypes were found out with frequency 0.0104～ 0.3021 . Hi Square test indicated the distribution of genotypes agreed with Hardy Weinberg equilibrium ( P > 0.05 ). Statistical analysis showed the followings:the heterozygosity ( H )>0.6 in each locus,the average polymorphism information content ( PIC )>0.7, Mean discrimination power ( DP )>0.8,probability of paternity exclusion ( EPP )>0.5,indicating that the STR markers used in the study were of great value in the researches of minority genetics. This not only founds the base for genetic structures of STR of Chinese but also provides valuable information for anthropogy,forensic medicine and ethnology.
基金
国家自然科学基金资助 (3 9970 40 1)~~
